Zebrafish danio rerio is a common research model in fish studies of toxicology, developmental biology, neurobiology and molecular genetics. Zebrafish models of human disease are widely used in many different fields of medical research, like. The zebrafish model nicely complements these deficiencies in mammalian experimental models. As a vertebrate that has many of the strengths of invertebrate model systems, it offers numerous advantages to researchers. Several pathological models have been established in adult zebrafish such as bone injury models. Jan 28, 2016 although they are primitive vertebrates, zebrafish danio rerio and medaka oryzias latipes have surpassed other animals as the most used model organisms based on their many advantages. The advantages of working with zebrafish in these areas are their small size, short generation time 1214 weeks and their capacity to produce. Zebrafish genomes contain a large amount of variation with 14.
This provides insight into the genetic control of developmental processes in humans because their genomes are similar. First used in the 1960s in biological research, zebrafish have proven to be excellent model organisms for developmental and disease studies. Download zebrafish books, the zebrafish has become one of the most important model organisms to study biologial processes in vivo. As the zebrafish has become an important model organism for the study of vertebrate development and disease, organ function, behavior, toxicology, cancer, and drug discovery, this book presents an important resource for future research. Zebrafish have many advantages as a model system for aging research. One promising model organism currently used is the zebrafish danio rerio. Pdf the zebrafish genetics and genomics download free. May 09, 2018 animals as model organisms, the silent sentinels, stand watch over the environmental health of the world. Jul 27, 2017 the zebrafish model organism database zfin. They are used in the laboratory to help scientists understand biological processes. These are nonhuman animal species which can be used to understand specific biological processes and to obtain informations which can provide an insight into working of other organisms.
Feb 01, 2014 one promising model organism currently used is the zebrafish danio rerio. Additionally, zebrafish are endowed with other advantages good for the study of organ regeneration. The zebrafish as a model for cancer disease models. To serve the zebrafish and wider biological research communities even better, we have recently expanded the options for searching, browsing, and downloading zfin data, and aim to facilitate the use of zebrafish as a model for human diseases. Environmental epigenetics, zebrafish, dna methylation, methylome, histone.
Among the model organisms, the zebrafish danio rerio is one of the best leading models to study. Pdf behavioral and neural genetics of zebrafish ebook. Studies on gene expression patterns, regulatory ciselements identification, and gene functions can be facilitated by using zebrafish embryos via a number of techniques, including transgenesis, in vivo. Zfin provides a wide array of expertly curated, organized and crossreferenced zebrafish research data. Introductionthe zebrafish has emerged as a model organism important for the identification and characterization of genes and pathways involved in development, organ function, behavior and disease. Model organisms have been used in various disciplines in order to understand different mechanisms underlying the problems. In conclusion, the zebrafish respond to edc at environmentally realistic concentrations. Zebrafish share a high degree of genetic and physiological similarity to mammals, including humans.
Classically, the zebrafish model organism has been used to elucidate the genetic and cellular mechanisms related to development since the embryo forms and grows externally following fertilization. This first volume provides stateoftheart descriptions of novel cellular imaging technologies and methods for culture of zebrafish stem cells, summarizes protocols for analyzingthe development of major organ systems including the central nervous system cns, and introduces the use of the zebrafish as a model system for human diseases. They are small in size, transparent, simple to handle, species with elevated fecundity, continuous reproduction and rapid. Interfacing chemistry, genetics and genomics pamela m. Application of this zebrafish partial lifecycle test on a chemically complex pulp mill effluent revealed both estrogenic and androgenic effects. Due to these features the zebrafish has become an important model organism to determine the in vivo function of a gene functional genomics during embryonic. Advantages of using zebrafish as a model organism andor. Mar 25, 2015 the zebrafish is a premier model organism for biomedical research, with a rich array of tools and genomic resources, and combining these with a fuller appreciation of wild zebrafish ecology could greatly extend its utility in biological research. Its emergence as a model organism for modern biological investigation began with the.
They experience senescence, have an evolutionarily conserved genome and are genetically manipulatable gerhard, 2003, lieschke and currie, 2007. The zebrafish danio rerio is a small tropical freshwater fish. Dec 22, 2010 the zebrafish is proving to be an extremely versatile new experimental model for unraveling the mysteries of innate immunity and has considerable promise as a system for the identification of novel modulators of this crucial biological process. Zebrafish model organism disease week arkana laboratories. Its use in modelling human disease has numerous advantages over other animals as zebrafish have a fully sequenced genome, high fecundity, short generation interval 34 months, rapid embryonic development, external fertilisation and translucent embryos. Zebrafish danio rerio is an ideal model organism for the study of vertebrate development. Aug 07, 2020 zebrafish grow at a tremendously fast rate as compared to other model organisms. Major technical advances have been essential for the generation of zebrafish cancer models relevant to human diseases. In particular, the zebrafish danio rerio has been established as one of the most important model organisms. Pdf zebrafish as a genetic model organism researchgate. As a vertebrate that has many of the strengths of invertebrate model systems, it offers numerous advantages to researchers interested in many aspects of embryonic development, physiology and disease. Native to south asia, it is a popular aquarium fish, frequently sold under the trade name zebra danio and thus often called a tropical fish although both tropical and subtropical the zebrafish is an important and widely used vertebrate model organism in scientific research, for. For more information click here the zebrafish information network zfin is the database of genetic and genomic data for the zebrafish danio rerio as a model organism.
Zebrafish as a model for human diseases tavakoli major. Adult zebrafish as a model organism for behavioural. Pdf the study of zebrafish as a leading model organism for developmental biology is rapidly expanding. Ijms free fulltext zebrafish as a model organism for the. Zebrafish as a model organism for genetic diseases youtube. It is also notable for its regenerative abilities, 4 and has been modified by researchers to produce many transgenic strains. Frontiers zebrafisha model organism for studying the. The developing embryo can be treated pharmacologically, providing an in vivo animal model for a high. Advantages of zebrafish usage in biomedical research. Since its emergence as an important model organism the molecular tools for studying zebrafish have been limited.
Although more difficult to manipulate than larvae, adult zebrafish display a full repertoire of mature behaviours making their characterisation. For decades, toxicology testing with animal models has been a crucial tool for studying the adverse health effects of chemicals and human disease. The zebrafish danio rerio an emerging model organism in the. Zebrafish as a model organism to study nanomaterial toxicity. The zebrafish model has recently emerged as a significant experimental system for developmental biology studies. The zebrafish is the canonical fish model for studying human disease. Zebrafish are vertebrates often used for in vivo studies, because they are relatively small in size and the embryonic and larval development is ex utero and fast, since within 5 days postfertilization dpf, all major organ systems are formed kimmel et al. Even though the zebrafish has been a model organism for decades and can now be found in over 1200 and counting. Transgenic zebrafish containing complex, patientrelevant genotypes have been used to. The zebrafish, danio rerio, is a fast expanding and extremely valuable model system for studying vertebrate developmental biology. Pdf on nov 27, 2018, farmanur rahman khan and others published zebrafish danio rerio as a model organism find, read and cite all the. Measuring zebrafish behaviour in zantiks unitsmodel.
This is due to the large clutches that each couple. The advantages of working with zebrafish in these areas are their small size, short generation time 1214. Although zebrafish danio rerio is a primitive vertebrate, it has several advantages over other model organisms the main reasons for it becoming an excellent model organism in different research fields are easy access to all stages of its body development, transparency of its embryos and larvaes, high genetic similarity, and. The importance of zebrafish in biomedical research universidade. Visual defects affect a large proportion of humanity, have a significant negative impact on quality of life, and cause significant economic burden.
Advantages of using zebrafish as a model organism andor learning centre. In zebrafish neurobehavioral protocols, experts in the field provide a series of detailed behavioral protocols dealing with the major current research areas in the field of behavioral neuroscience. A ratelimiting factor, however, is the mechanical stimulus required to induce the inflammatory response. Zebrafish are rapidly becoming a leading model organism for cancer research. Heather flanagansteet, provides an overview of the use of zebrafish as a model organism for genetic research.
Vertebrate animal models are used because of the conservation that exists between humans and other vertebrates in the genetics, development, and metabolic. It is smallsized fish needs less space for residence. First established as a valuable vertebrate model system for studying development, zebrafish have emerged as an attractive animal system for modeling human cancers. Oct 29, 2020 the zebrafish, danio rerio, is a fast expanding and precious model system for studying many aspects of cell biology within an intact vertebrate organism. The zebrafish danio rerio as a model organism, with emphasis on. The zebrafish liver can also be used to study drug toxicity, however in comparison to other organs, the zebrafish model. Why use zebrafish as a model organism for research work.
Combining this with precise genome editing, it is not so hard to imagine that zebrafish could play a large role in personalized and precision medicine. Using zebrafish as the model organism to understand organ. Zebrafish are used to study development, toxicology and toxicopathology, specific. Zfin continuously assesses trends in zebrafish research, adding new data types and providing data repositories and tools that members of the research community can use to navigate. Jan 01, 2003 the zebrafish has emerged as a model organism important for the identification and characterization of genes and pathways involved in development, organ function, behavior and disease. The zebrafish is an important and widely used vertebrate model organism in scientific research, for example in drug development, in particular preclinical development. Recently, zebrafish became a popular model organism for the deep understanding of regeneration based on its powerful regenerative capacity, in particular the organs that are limitedly regenerated in mammals. Zebrafish have the exceptional ability to repair and regeneration power of muscle. Pdf zebrafish danio rerio as a model organism researchgate. Zebrafish as a developmental model organism for pediatric.
Zon adopted the zebrafish as a model organism largely because fertilization occurs exogenously. The wide variety of visual disorders and the large number of gene mutations responsible require a flexible animal model system to carry out research for possible causes and cures for the blinding conditions. Wellestablished zebrafish assays have frequently been utilised for measurement of cardiac function, cns assessment, gastrointestinal function and developmental toxicity 9, 10. Feb 26, 2019 download pdf copy by hannah simmons, m. From this point of view, the zebrafish has become a favorite model organism in different scientific research fields in recent years because of its rapid embryonic development, transparency of its embryos, and its large number of offspring along with several other advantages. As a model organism, zebrafish danio rerio offer an opportunity to perform steadfast scientific investigations in a robust and highthroughput manner.
Jan 21, 2021 zebrafish as a model organism for developmental disorders. Zebrafish has been used for many years as a model to study development and disease. Among them, zebrafish has special characteristics, which expedite its use as a model organism. Zebrafish also display a range of behavioural phenotypes that resemble aspects of human disease kalueff et al. The zebrafish pdf download full pdf read book page. Zebrafish as a model organism for nutrition and growth. Dec 16, 2020 zebrafish is increasingly used as a genetic model organism for aquaculture species and in to xicogenomics and also to genera te zebr afish disease m odels f or application in human biom edicines. Zebrafish danio rerio as a model organism intechopen.
This primer will provide a synopsis of the different cancer models generated by the zebrafish community to date. The zebrafish liver can also be used to study drug toxicity, however in comparison to other organs, the zebrafish model of liver toxicity has. The zebrafish danio rerio is a freshwater fish belonging to the minnow family of the order cypriniformes. Lipid metabolism and signaling in zebrafish shiuying ho, steven a. The zebrafish offers a number of features, such as its rapid development, tractable genetics, suitability for in vivo imaging and chemical screening, that make it an attractive model to cancer researchers. Furthermore, zebrafish is the only vertebrate model organism that has the potential for highthroughput screening for toxic and therapeutic compounds. Aug 02, 2010 the general suitability of zebrafish as a model organism, as well as its use in the genetic and neuroanatomical analysis of larval behaviour has been comprehensively described elsewhere 1, 2. Danio rerio zebrafish is an excellent model organism for cnv studies. Zebrafish danio rerio as a model for the study of aging. Adult zebrafish as a model organism for behavioural genetics. Zebrafish zantiks units automate model organism behavioral. Because its introduction as a modern experimental model organism.
Zebrafish as a model organism for the development of. A model organism often shortened to model is a nonhuman species that is extensively. The genetic pathways driving cancer are highly conserved between zebrafish and humans, and the ability to easily manipulate the zebrafish genome to rapidly generate transgenic animals makes zebrafish an excellent model organism. Danio rerio zebrafish is an elective model organism for the study of vertebrate development because of. C haracteristics of z ebrafish as a model organism.
Zebrafish danio rerio as a model for the study of aging and. The development of animal models has allowed a better understanding of. The results support that zebrafish is a suitable model. These models develop tumors in various organ sites that bear striking resemblance to human malignances, both. The ability of zebrafish to produce thousand of embryos in a synchronous manner has made zebrafish an invaluable tool for genetic and chemical screens. The zebrafish, danio rerio a tropical and small freshwater fish, is an early vertebrate model organism. Also, unlike other animal models, the genes of zebrafish can be manipulated. The zebrafish as a model organism for evaluation of.
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